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Rapid determination of domoic acid in seafood by fluorescence polarization immunoassay using a portable analyzerстатья
Информация о цитировании статьи получена из
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 5 июня 2024 г.
- Авторы: Ogura Yu, Fukuyama Mao, Kasuya Motohiro, Shigemura Koji, Eremin Sergei A., Tokeshi Manabu, Hibara Akihide
- Журнал: Analytical Sciences
- Том: 39
- Номер: 12
- Год издания: 2023
- Издательство: Japan Society for Analytical Chemistry
- Местоположение издательства: Japan
- Первая страница: 2001
- Последняя страница: 2006
- DOI: 10.1007/s44211-023-00413-6
- Аннотация: Monitoring phycotoxin accumulation in marine products such as edible shellfish is a regulatory requirement in many countries. Therefore, a simple and rapid onsite quantification method is sought. Herein, we present a fluorescence polarization immunoassay (FPIA), a well-known one-step immunoassay, using a portable fluorescence polarization analyzer for domoic acid (DA), widely referred to as the primary toxin of amnesic shellfish poisoning (ASP). To establish FPIA for DA, the matrix effect of methanol, which is widely used to extract DA from shellfish, on FPIA was investigated. To validate this method, we performed a spike recovery test using oysters containing DA at a concentration equivalent to the regulatory limits of North America and the European Union (20 mg/kg). The recovery rate was found to be 79.4–114.7%, which is equivalent to that of the commercially available enzyme-linked immunosorbent assay (ELISA). We expect that this FPIA system will enable the quantitative onsite analysis of DA and significantly contribute to the safety of marine products.
- Добавил в систему: Мухаметова Лилия Инилевна