Comparative proteome mapping of tumor stem cells isolated from U87 glioblastoma, neural stem and multipotent mesenchymal stromal cells of a human: from cataloguing of cell proteins to novel paradigm of proteome-based cell therapy of tumors

Bryukhovetskiy, A.S.; Shevchenko, V.E.; Chekhonin, V.P.; Bryukhovetskiy, I.S.; Kovalev, S.V.; Baklaushev, V.P.; Davydov, M.I.

Авторы: Bryukhovetskiy A.S., Shevchenko V.E., Chekhonin V.P., Bryukhovetskiy I.S., Kovalev S.V., Baklaushev V.P., Davydov M.I.
Журнал: Cellular Transplantation & Tissue Engineering
Том: 8
Номер: 2
Год издания: 2013
Первая страница: 84
Последняя страница: 91
Аннотация: We performed proteome mapping, cataloguing and bioinformation analysis of protein lysates of human neural (CD133+) stem cells (NSC) isolated from olfactory sheath of a nose, multipotent mesenchymal (CD29+, CD44+, CD73+, CD90+, D34-) stromal cells (MMSC) isolated from human bone marrow and tumor (CD133+) stem cells (TSC) isolated from the human U87 glioblastoma cell line. We identified 1664 proteins in the examined lysates of stem cells (SC), 1052 (63.2%) of which are identical in NSC and TSC and 607 proteins (36.47%) are identical in MMSC and TSC. Other proteins in U87 glioblastoma TSC are oncospecific or carcinogenesis associated. The biological processes, molecular functions, cell localization and protein signal pathways of the proteins available in all three proteomes were annotated by PubMed, PANTHER, Gene Ontology and KEGG databases. It was shown that gliomaspheres of U87 glioblastoma had only 10 intracellular pathways of signal transduction (IPST) that were not modified by neoplastic process, but only two of them (integrin and focal adhesion pathways) were accessible for regulatory action on gene candidates in TSC nucleus. Carcinogenesis free membrane proteins, intracellular pathways of signal transduction and genes expressing proteins of these pathways in U87 glioblastoma TSC can be viewed as main targets for regulatory effect on TSC. We offer a novel concept of proteome-based complex therapy of tumors.
Добавил в систему: Ковалев Сергей Васильевич

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Comparative proteome mapping of tumor stem cells isolated from U87 glioblastoma, neural stem and multipotent mesenchymal stromal cells of a human: from cataloguing of cell proteins to novel paradigm of proteome-based cell therapy of tumorsстатья

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