Assessment of the Europium(III) Binding Sites on Albumin Using Fluorescence Spectroscopyстатья
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Дата последнего поиска статьи во внешних источниках: 2 сентября 2014 г.
Аннотация:Intrinsic fluorescence quenching of bovine serum
albumin (BSA) and europium(III) luminescence in BSA complexes
were investigated. The number of BSA binding sites (n) and
equilibrium constant (Keq) values were determined from both
measurements provided qualitatively different results. While the
modified Stern−Volmer relation for BSA fluorescence quenching
gave n = 1 at pH 4.5 and pH 6, two sets of binding sites were
determined from Eu3+ luminescence with n1 = 2, n2 = 4 at pH 6 and
n1 = 1, n2 = 2 at pH 4.5. The model explaining the discrepancy
between the results obtained by these fluorescent approaches was
suggested, and the limitations in application of the “log−log” Stern−
Volmer plots in analysis of binding processes were discussed.