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Gammaproteobacterial Methanotrophs Dominate Cold Methane Seeps in Floodplains of West Siberian Riversстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 22 октября 2014 г.
- Авторы: Oshkin I.Y., Wegner C.E., Lüke C., Glagolev M.V., Filippov I.V., Pimenov N.V., Liesack W., Dedysh S.N.
- Журнал: Applied and Environmental Microbiology
- Том: 80
- Номер: 19
- Год издания: 2014
- Издательство: American Society for Microbiology
- Местоположение издательства: United States
- Первая страница: 5944
- Последняя страница: 5954
- DOI: 10.1128/AEM.01539-14
- Аннотация: A complex system of muddy fluid-discharging and methane (CH4)-releasing seeps was discovered in a valley of the river Mukhrinskaya, one of the small rivers of the Irtysh Basin, West Siberia. CH4 flux from most (90%) of these gas ebullition sites did not exceed 1.45 gCH4/h, while some seeps emitted up to 5.54 gCH4/h. The δ13C value of methane released from these seeps varied between −71.1 and −71.3‰, suggesting its biogenic origin. Although the seeps were characterized by low in situ temperatures (3.5 to 5°C), relatively high rates of methane oxidation (15.5 to 15.9 nmolCH4/ml/day) were measured in mud samples. Fluorescence in situ hybridization detected 10 000 000 methanotrophic bacteria (MB) per g of mud (dry weight), which accounted for up to 20.5% of total bacterial cell counts. Most (95.8 to 99.3%) methanotroph cells were type I (gammaproteobacterial) MB. The diversity of methanotrophs in this habitat was further assessed by pyrosequencing of pmoA genes, encoding particulate methane monooxygenase. A total of 53,828 pmoA gene sequences of seep-inhabiting methanotrophs were retrieved and analyzed. Nearly all of these sequences affiliated with type I MB, including the Methylobacter-Methylovulum-Methylosoma group, lake cluster 2, and several as-yet-uncharacterized methanotroph clades. Apparently, microbial communities attenuating methane fluxes from these local but strong CH4 sources in floodplains of high-latitude rivers have a large proportion of potentially novel, psychrotolerant methanotrophs, thereby providing a challenge for future isolation studies.
- Добавил в систему: Глаголев Михаил Владимирович