Cationized Extracellular Vesicles for Gene Delivery

Klyachko, N.L.; Haney, M.J.; Lopukhov, A.V.; Le-Deygen, I.M.

Авторы: Klyachko Natalia L., Haney Matthew J., Lopukhov Anton V., Le-Deygen Irina M.
Журнал: Scientific reports
Том: 14
Год издания: 2024
Издательство: Nature Publishing Group
Местоположение издательства: United Kingdom
Первая страница: 1
Последняя страница: 13
Номер статьи: 25818
DOI: 10.1038/s41598-024-75985-y
Аннотация: Klyachko, N.L., Haney, M.J., Lopukhov, A.V., Le-Deygen, I.M. (2024). Cationized extracellular vesicles for gene delivery. Sci Rep. V. 14. Art N 25818. P. 1-13. IF 4.3 (Q1) https://doi.org/10.1038/s41598-024-75985-y. Published 28 October 2024Last decade, extracellular vesicles (EVs) attracted a lot of attention as potent versatile drug delivery vehicles. We reported earlier the development of EV-based delivery systems for therapeutic proteins and small molecule chemotherapeutics. In this work, we first time engineered EVs with multivalent cationic lipids for the delivery of nucleic acids. Stable, small size cationized EVs were loaded with plasmid DNA (pDNA), or mRNA, or siRNA. Nucleic acid loaded EVs were efficiently taken up by target cells as demonstrated by confocal microscopy and delivered their cargo to the nuclei in triple negative breast cancer (TNBC) cells and macrophages. Efficient transfection was achieved by engineered cationized EVs formulations of pDNA- and mRNA in vitro. Furthermore, siRNA loaded into cationized EVs showed significant knockdown of the reporter gene in Luc-expressing cells. Overall, multivalent cationized EVs represent a promising strategy for gene delivery.
Добавил в систему: Ле-Дейген Ирина Михайловна

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