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Varieties of interactions of anti-CD133 aptamers with cell cultures from patient glioblastomaстатья
- Авторы: Antipova Olga, Moiseenko Valeria, Dzarieva Fatima, Savchenko Ekaterina, Pronin Igor, Pavlova Galina, Kopylov Alexey
- Журнал: SLAS DISCOVERY
- Том: 29
- Номер: 8
- Год издания: 2024
- Номер статьи: 100195
- DOI: 10.1016/j.slasd.2024.100195
- Аннотация: Development of aptatheranostics for glioblastoma (GB) requires investigating aptamer interactions with cells.The paper has described flow cytometry (FC) assessment of direct interactions of fluorescent anti-CD133aptamers with cells, focusing on cell cultures derived from patient GB (CCPGB). Conventional cell lines withdifferent levels of CD133 mRNA, Caco-2 and HCT116, were used to compare interactions with known 2′FY-RNAaptamer A15 and DNA aptamers of Ap and Cs series, labeled with FAM and Cy5. In addition, interactions ofcertain non-aptameric oligonucleotides were studied. In the case of antibody interactions with cells, FC signals,mean fluorescence intensities (MFIs), correlated with sizable amounts of CD133 mRNA in Caco-2 cells, andCCPGBs 107 and G01. Unexpectedly, MFI per se could not be the solid indicator of specific interactions ofaptamer - CD133/cell. Instead, two types of interactions, target CD133-driven and off-target membrane-asso-ciated ones, contribute to MFI. The latter was notably observed for CCPGB Sus/fP2 with tiny CD133 mRNAamount. To prove specificity of aptamer - CD133/cell interactions, titration experiments have been performed,revealing half-saturation concentrations of 120±27 for 2′FY-RNA A15 and 180±12 for DNA Cs5 with Caco-2cells. This knowledge is an essential step to develop aptatheranostics for GB.
- Добавил в систему: Антипова Ольга Михайловна
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Полный текст | PIIS2472555224000571.pdf | 2,5 МБ | 2 марта 2025 [anna_bacheva] |