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Separation of Short Fluorescently Labeled Peptides by Gel Electrophoresis for an In Vitro Translation Studyстатья
- Авторы: Tolicheva O.A., Bidzhieva M.S., Kasatskiy P.S., Marina V.I., Sergiev P.V., Konevega A.L., Paleskava A.
- Журнал: Nanobiotechnology Reports
- Том: 19
- Номер: 3
- Год издания: 2024
- Издательство: Pleiades Publishing, Ltd
- Местоположение издательства: Road Town, United Kingdom
- Первая страница: 423
- Последняя страница: 431
- DOI: 10.1134/s263516762460127x
- Аннотация: The development of simple, readily available, and sensitive methods for studying bacterial protein synthesis is an important fundamental and applied task. We recently demonstrated the possibility of visualizing short BODIPY-labeled phenylalanine- and leucine-containing peptides using urea-polyacrylamide gel electrophoresis under denaturing conditions. In this work, we expand the range of test sequences and included peptides with valine, lysine, and the modified amino acid εNH2-DOTA-lysine. The described method can also be used to study the mechanism of translation inhibition, as shown by elaboration of the specific action of the antibiotics etamycin A and viomycin.
- Добавил в систему: Марина Валерия Ивановна
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Полный текст | 4_Separation_of_Short_Fluorescently_Labeled_Peptides_by_Gel… | 1009,0 КБ | 19 декабря 2024 [MarinaValeriya] |