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Polymorphism of the ITS1 and ITS2 of nuclear ribosomal DNA in Paeonia lactiflora Pall. (Paeoniaceae) determined using next-generation sequencing technologyстатья
- Авторы: Efimov S.V., Degtjareva G.V., Terentieva E.I., Samigullin T.H., Logacheva M.D., Valiejo-Roman C.M.
- Журнал: Skvortsovia
- Том: 4
- Номер: 1
- Год издания: 2017
- Издательство: Ботанический сад УрО РАН
- Местоположение издательства: Екатеринбург
- Первая страница: 6
- Последняя страница: 7
- Аннотация: The genus Paeonia L. constitutes a convenient case study of reticulate evolution. The hybrid species are mostly identified by full or partial ITS sequence additivity of the putative parental species (Sang et al., 1995, 1997; Punina et al., 2012). One of the few species whose hybridogeneous nature has not been demonstrated is Paeonia lactiflora Pall. distributed in China, Mongolia, and in the adjacent Russian territory. This diploid species is quite distinct morphologically. The geographical area of P. lactiflora overlaps with that of P. veitchii Lynch in the south and with species from the P. obovata Maxim. complex in the east. Hybrids between these species have not been recorded in natural habitats. Yet there is a cultivar Paeonia 'Fan Tan,' a putative hybrid P. lactiflora Ч P. obovata (Smirnow, 1975). Recently, there appeared new data on the hybrid nature of P. anomala L. (P. lactiflora Ч P. veitchii) (Pan et al., 2007). The next-generation sequencing approach has brought about new technical opportunities for obtaining ITS haplotypes of nuclear rDNA for a representative sample that would include rare variants. We have determined ITS1 and ITS2 sequences using the Illumina HiSeq 2000 platform (USA) with read length of 101 bp from the terminus of each fragment. The alignment of the sequence reads with the assembled ribosomal operon has allowed us to reveal random polymerase errors along with polymorphic positions. A total of 13 polymorphic positions have been found: 8 in ITS1 (69, 93, 117, 125, 145, 158, 212, and 217) and 5 in ITS2 (25, 35, 90, 189, and 199). Polymorphism at these positions, (except for 125, 145 and 217) is not of hybridogeneous origin but may be explained by the presence of additive nucleotides not registered in other species. We assume that the observed additive polymorphism of nuclear ribosomal transcribed spacers could be attributed to the genetic differentiation within the species.
- Добавил в систему: Дегтярева Галина Викторовна