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Zinc-dependent Nucleosome Reorganization by PARP2статья
- Авторы: Maluchenko Natalya, Saulina Alexandra, Geraskina Olga, Kotova Elena, Korovina Anna, Feofanov Alexey, Studitsky Vasily
- Журнал: bioRxiv (The preprint server for biology)
- Год издания: 2023
- DOI: 10.1101/2023.10.17.562808
- Аннотация: Poly(ADP-ribose)polymerase 2 (PARP2) is a nuclear protein that acts as a DNA damage sensor; it recruits the repair enzymes to a DNA damage site and facilitates formation of the repair complex. Using single particle Förster resonance energy transfer microscopy and electrophoretic mobility shift assay (EMSA) we demonstrated that PARP2 forms complexes with a nucleosome containing different number of PARP2 molecules without altering conformation of nucleosomal DNA both in the presence and in the absence of Mg 2+ or Ca 2+ ions. In contrast, Zn 2+ ions directly interact with PARP2 inducing a local alteration of the secondary structure of the protein and PARP2-mediated, reversible structural reorganization of nucleosomal DNA. AutoPARylation activity of PARP2 is enhanced by Mg 2+ ions and modulated by Zn 2+ ions: suppressed or enhanced depending on the occupancy of two functionally different Zn 2+ binding sites. The data suggest that Zn 2+ /PARP2-induced nucleosome reorganization and transient changes in the concentration of the cations could modulate PARP2 activity and the DNA damage response.
- Добавил в систему: Малюченко Наталия Валериевна