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Changes in Physicochemical Properties of HRP Induced by AC Electromagnetic Fields of Commercial Frequencyстатья
- Авторы: Shumov Ivan, Tatur Vadim, Lukyanitsa Andrei, Ziborov Vadim, Ivanov Yuri
- Журнал: Preprints
- Год издания: 2025
- Первая страница: 1
- Последняя страница: 12
- DOI: 10.20944/preprints202503.0577.v1
- Аннотация: Low‐frequency electromagnetic fields, induced by alternating current, are known to influence physicochemical properties and functioning of enzymes, including their catalytic activity. Herein, by using atomic force microscopy (AFM) and spectrophotometry analysis in parallel, we have investigated how the incubation near an autotransformer operated at 50 Hz influences the physicochemical properties of horseradish peroxidase (HRP). We have found that 30 min incubation of the enzyme above the coil of a loaded autotransformer enhances a disaggregation of HRP on mica and the number of adsorbed enzyme particles by two orders of magnitude in comparison with the control sample. And most interestingly, the incubation of HRP above the switched‐off transformer for the same period of time has been found to cause a disaggregation of the enzyme, An increase in the activity of HRP against ABTS has been observed in the both cases. We hope that the interesting effects reported will emphasize the importance of consideration of the influence of low‐frequency electromagnetic fields on enzymes in the design of laboratory and industrial equipment intended for operation with enzyme systems.
- Добавил в систему: Лукьяница Андрей Александрович