Sample Stacking and On-line Derivatization for the Analysis of Ampicillin and Amoxicillin by Microemulsion Electrokinetic Chromatographyстатья
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Дата последнего поиска статьи во внешних источниках: 2 апреля 2015 г.
Аннотация:A novel method using microemulsion electrokinetic chromatography (MEEKC) combining the preconcentration technique called reversed
electrode polarity stacking mode (REPSM) with subsequent on-line derivatization has been developed for the quantitative determination of
ampicillin and amoxicillin. It was found that the derivatization of antibiotics with 2,3-naphtalenedicarboxaldehyde (NDA) is accelerated
greatly in microemulsion media, compared to the standard derivatization procedure, no heating being required. Various parameters that
affect the ratio of derivatization, such as temperature, mixing and reaction time, concentration of NDA solution, and plug length were
investigated and optimized. The combination of RESPM technique with the following derivatization allows the detection limits of
ampicillin and amoxicillin to be decreased by a factor of 300 or more. The potential of the developed method was demonstrated by
the determination of these antibiotics in a biological sample through the analysis of urine.