Effect of trehalose on oxygen evolution and electron transfer in photosystem 2 complexesстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 23 июня 2015 г.
Аннотация:The pigment–protein complex of photosystem 2 (PS 2) catalyzes the lightdriven oxidation of water molecule and the reduction of plastoquinone. In this work, we studied the effect of the disaccharide trehalose, which is unique in its physicochemical properties, on isolated PS 2 complex. It was found that trehalose significantly stimulated the steadystaterate of oxygen evolution. The study of single flashinduced fluorescence decay kinetics demonstrated that trehalose did not affect the rate of QA– oxidation, although it led to an increase in the relative fractions of PS 2 reaction centers capable of QA– oxidation. Trehalose also prevented PS 2 complexes from being inactivated on prolonged storage. We propose that in the presence of trehalose, which affects the extent of hydration, the protein can preferentially exist in a more optimal confor mation for effective functioning.