Inactivation of Planktonic Escherichia Coli by High Intensity Focused Ultrasound Pulses

Matula, T.; Brayman, A.; MacConaghy, B.; Wang, Y.; Chan, K.; Monsky, W.; Chernikov, V.; Buravkov, S.; Khokhlova, V.

Авторы: Thomas Matula, Andrew Brayman, Brian MacConaghy, Yak-Nam Wang, Keith Chan, Wayne Monsky, Valery Chernikov, Sergey Buravkov, Vera Khokhlova
Сборник: Abstract book of International Congress on Ultrasonics (ICU 2017, December 18-20, Honolulu, USA)
Тезисы
Год издания: 2017
Первая страница: 136
Последняя страница: 136
Аннотация: This study addresses inactivation of E. coli in vitro using pulsed ultrasound and cavitation as a first step toward noninvasive treatment of human abscesses. Cells were cultured in tryptic soy broth until a cell concentration of about 1 x 10^9 cells/mL was achieved. The cultures were removed from the incubator and allowed to stand at room temperature as 5 or 10 mL aliquots were removed and transferred to thecorresponding exposure vessel. The cell suspensions were exposed to 1-MHz pulsed HIFU (10 cycles, 2 kHz repetition frequency, +65/-12.8 MPa focal pressures, +65 MPa shock amplitude). Studies were performed with 0, 1, 2, 5, 1, and 20 minute exposures. Surviving fraction was assessed by coliform assay. Inactivation kinetics were curvilinear; the time course of bacterial inactivation slowed as sample volume increased. Inactivation of 50% cells was achieved in 2.5 or 6 minutes for 5 or 10 ml samples, respectively, as compared to about 1.2 minutes for 0.1 mL samples from an earlier study. Corresponding inactivation rates were 42, 1000, or 833 million cells/min for the 0.1, 5, or 10 mL treatments, respectively. Surviving cells from 5 min exposures of both 5 and 10 ml samples appeared normal under light microscopy, with minimal debris; after 20 min, debris dominated. TEM images of insonated samples showed some undamaged cells, a few damaged but largely intact cells, and comminuted debris. TEM results showed that the rate of HIFU-induced bacterial inactivation is much greater than the microscopic appearance would suggest as cellular damage associated with substantive but incomplete levels of inactivation can be subtle. This study demonstrates the potential of using HIFU for disinfection of abscesses without the need for catheter drainage. This research was supported by NIH 5R01EB019365-02, 2R01EB007643-09, and RFBR 16-02-00653.
Добавил в систему: Хохлова Вера Александровна

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