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Heterologous strategy enhancing the sensitivity of the fluorescence polarization immunoassay of clinafloxacin in goat milkстатья
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или Scopus- Авторы: Chen J., Shanin I.A., Lv S., Wang Q., Mao C., Xu Z., Sun Y., Wu Q., Eremin S.A., Lei H.
- Журнал: Journal of the Science of Food and Agriculture
- Год издания: 2015
- Издательство: John Wiley & Sons Inc.
- Местоположение издательства: United States
- DOI: 10.1002/jsfa.7228
- Аннотация: Clinafloxacin is used for the treatment of disease in food-producing animals, e.g., Brucella melitensis, which often occurs in goats; however, the clinafloxacin residue in goat milk may harm human health and result in the development of drug-resistant bacterial strains or allergies. Despite this, there is not a rapid, sensitive and accurate analytical method in goat milk for a rapid screening or monitoring purpose.|One homologous and five heterologous tracers were designed and compared for fluorescence polarization immunoassay(FPIA) optimization. Based on the combination of a heterologous tracer (PAZ-FITC, synthesized with pazufloxacin and FITC) and the antibody against clinafloxacin, a highly sensitive FPIA was established for the detection of clinafloxacin residue in goat milk for the first time. The IC50 value was 29.3 µg/L for clinafloxacin in the heterologous format, 6 times lower than that of the combination of the homologous tracers and the antibody. The recoveries ranged from 86.8% to 104.5%, with the relative standard deviation ranging from 4.1% to 7.2%. Validation by high-performance liquid chromatography (HPLC) confirmed that the results obtained from the proposed FPIA were in agreement with those of HPLC.|This proposed heterologous strategy for enhanced FPIA is sensitive and rapid enough for the high-throughput detection of clinafloxacin residue in goat milk.
- Добавил в систему: Шанин Илья Александрович